Brin-bd11
WebThese results agree with rat insulin mRNA expression in rat islets, INS-1E and BRIN BD11 cells [11]. (iii) RIA provided significantly higher insulin concentrations than IR binding assay in islets ... WebApr 11, 2024 · the BRIN-BD11 pancreatic beta cell line in . vitro. The effect of the extract was found to be . glucose-independent [56]. Salacia reticulata . var. b-Diandra Weight (Family, C elestraceae)
Brin-bd11
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WebMar 3, 2024 · BRIN-BD11 cells were treated with HWHF and changes in membrane potential and intracellular [Ca 2+]i; were measured using FLIPR membrane potential and calcium assay kits (Molecular Devices, Sunnyvale, CA, USA) according to the manufacturer instructions . BRIN-BD11 cells were seeded in 96 well plates, and left to attach overnight … WebApr 11, 2024 · (A) BRIN‐BD11 cells were cultured (24 h) with test peptides (10 −8 and 10 −6 M) and proliferation detected using Ki-76 staining. (B) BRIN‐BD11 were cultured (24 h) with test peptides (10 −8 and 10 −6 M) in the presence of a cytokine cocktail and apoptosis detected using the TUNEL assay. All values are mean ± SEM (n=3).
WebAug 14, 2024 · The PTD method showed good efficiency only in BRIN-BD11 cells (68%), whereas it failed in PANC-1 and HEK293 cells. By contrast, the syn-mRNA method was highly effective in all three cell types (29–71%). We conclude that using synthetic mRNA is a more robust and less labor-intensive approach than using the PTD-fusion alternative. WebDownload scientific diagram Proposed model for GLP-1R trafficking and its contribution to GSIS in cultured BRIN-BD11 cells. GLP-1R is trafficked to lysosome after internalization. GLP-1R ...
WebMay 25, 2024 · The current study was aimed at highlighting the role of blood pancreatic amylase in the regulation of glucose homeostasis and insulin secretion in a porcine … WebDec 22, 2024 · MS—275 alters transcriptome profile in BRIN-BD11 pancreatic beta cells. (A) Volcano plot from hierarchical clustering of differentially expressed genes on MS-275 treatment in BRIN-BD11 pancreatic beta cells. The log 2 fold change is represented in the x-axis, whereas –log 10 of the corrective p-value is represented in the y-axis. Red dots ...
WebJul 7, 2009 · BRIN-BD11 cells also showed pronounced insulin responses to leucine, KIC, L-arginine, L-alanine and palmitic acid. All the cell lines tested gave significant responses to the neurotransmitters carbachol and glibenclamide with increased insulin release. A comparison was made between the functional characteristics of the various cell lines with ...
WebMoreover, the mode of action of UJ was analyzed in palmitate (PA)-induced steatotic human HuS-E/2 hepatocytes and in hyperglycemia-simulating rat BRIN-BD11 pancreatic β cells. In PA-induced steatotic human hepatocytes, UJ treatment promoted lipid clearance via pAMPK, pACC and CPT-1 upregulation and SREBP-1c downregulation. new orleans charity hospital renovationWebAug 1, 2004 · Briefly, BRIN BD11 cells were incubated at 37°C, for 2 h with either FLIPR membrane potential assay kit or FLIPR Ca 2+ assay kit (Molecular Devices, Sunnyvale, USA), to a final volume of 200 μL new orleans charities listWebJan 1, 2006 · The stimulus-secretion coupling for hypotonicity-in-duced insulin release was investigated in BRIN–BD11 cells. A 50 mM decrease in extracellular NaCl caused a twofold increase in insulin release. new orleans charismaticWebOct 5, 2024 · Long term exposure of the BRIN-BD11 cells to CPE enhances cell number and may protect against palmitate and proinflammatory cytokines by activating multiple pathways. Thus, the current study suggests that the possible antidiabetic properties of CPE may be linked to enhanced GLP-1 secretion and β-cell protection which could be … introduction to lakes onlineWebDec 11, 2024 · The BRIN-BD11 cell line offers an easy-to-utilize, cell-based system that has been reported to provide a stable, reproducible, and robust system for the study of … new orleans charter bus serviceWebMay 14, 2024 · For all experiments, BRIN-BD11 cells were incubated under test conditions for a 20-minute period at 37°C, with the resulting supernatant collected and insulin content assessed by radioimmunoassay . Acute In … introduction to labor lawWebSep 1, 2002 · The BRIN-BD11 cells were harvested and seeded into 24-multiwell plates at a density of 5 × 10 5 per well and allowed to attach overnight at 37°C. Standard RPMI-1640 tissue culture medium containing 11.1 mmol/l glucose, which was routinely used to culture BRIN-BD11 cells, was removed from each well and replaced with culture media … new orleans charity hospital today